Detection of Vibrio anguillarum by Dot Blot Hybridization with Digoxigenin Labeled Probe by PCR

A digoxigenin labeled DNA probe was produced by PCR method using toxR gene between 366 bp and 571 bp of Vibrio anguillarum as a target.The length of probe was 206 bp.Together with two V.anguillarum strains,ten pathogenic vibrio strains were chosen to detect the specificity of probe for V.anguillarum by dot blot hybridization.Result showed that the probe was able to hybridize with V.anguillarum.And no hybridization signals were observed using other eight vibrio strains most close to V.anguillarum,which demonstrated that the probe had strong specificity and had high value in detecting V.anguillarum.