Hormone responses to sedative drugs and cold exposure in two rat lines with high and low alcohol sensitivity

Abstract Two rat lines bred for differences in motor impairment in the tilting plane test after a moderate dose of ethanol were compared for peripheral hormone responses. The alcohol-sensitive ANT rats had significantly lower plasma corticosterone concentrations than the alcohol-insensitive AT rats 30 min after an IP saline injection. Ethanol (2 g/kg, IP) and lorazepam (3 mg/kg, IP) injections increased the corticosterone concentration in ANT rats. Sodium barbital (160 mg/kg, IP) did not produce any increase in these rats; instead, it prevented any increase caused by a tilting plane test procedure 10 min before decapitation. Three trials on the tilting plane significantly elevated the corticosterone concentration in saline-treated ANT rats, but produced no additional increase in drug-treated ANT rats. In AT rats, drug injections caused no significant corticosterone increase but the tilting plane test procedure after barbital (lorazepam) treatment(s) elevated the corticosterone concentration. Cold exposure (+4°C for 30 min) of the drug-naive animals elevated their concentrations of serum and adrenal corticosterone, thyrotropin, and growth hormone, but not of prolactin and luteinizing hormone. The increase in serum corticosterone was greater in AT than ANT rats, whereas the increase in serum thyrotropin was slightly greater in ANT rats. No differences between the rat lines were found in the growth hormone, prolactin, and luteinizing hormone levels. The results confirm and extend our earlier findings of the inability of ANT rats to produce additional stress responses to behavioral challenges when being intoxicated by sedative drugs, which may at least partly account for their increased sensitivity to sedative drugs.