PERBANDINGAN UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK BIJI PEPAYA (Carica papaya L.) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Methicillin Resistant Staphylococcus aureus (MRSA) SECARA IN VITRO
2017
Methicillin Resistant Staphylococcus aureus (MRSA) is one of
resistance problem, causing by Staphylococcus aureus, which can cause several
infection. An alternative antibiotic should be examined to reduce the incidence of
resistance. Indonesia produce tons of papaya (Carica papaya L. ) each year and
waste of the seeds are disused. Therefore, researcher examined the benefit of papaya
seeds extract against both of bacteria. Objective: Comparing antibacterial activity of
papaya seeds extract towards Staphylococcus aureus and MRSA. Methods:
Antibacterial activity was tested by using dilution method to find out minimum
inhibitory concentration (MIC). Each bacteria gains seven different concentrations,
100%, 50%, 25%, 12,5%, 6,25%, 3,125%, and 1,5625%. MBC was determined by
plating tube suspension from broth dilution test on nutrient agar plate. Results:
After 4 time repetition, MIC of papaya seeds extract that inhibited the growth of
Staphylococcus aureus was 25%, meanwhile toward MRSA was 50%. Concentration
of papaya seeds extract that needed to inhibit Staphylococcus aureus is lower than
MRSA. The colony of Staphylococcus aureus and MRSA were found at all
concentrations in all nutrient agar plate. Conclusions: There is a bacteriostatic
activity in papaya seeds extract against the growth of Staphylococcus aureus with
MIC of 25% and MRSA with MIC of 50%. There is no bactericidal activity in
papaya seeds extract against Staphylococcus aureus and MRSA.
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