Research Article Optimization, Production and Characterization of Multidrug Resistant Cultures Isolated from Hospital Premises

The present study is carried out by isolation of Multidrug resistant culture from hospital premises. The multidrug resistant cultures showed growth in the presence of multiple antibiotics. In this work the 4 soil samples were used and out of 4 total 5 cultures were isolated but only 2 cultures were characterized through Bergey’s manual. The cultures were S.equisimilis and N.sicca and antibiotics were Oofloxacin, Erythromycin, Floxip, Trimexazole, Ampilox, Cephalaxin and Almox. The Oofloxacin showed best resistant activity against 2 bacterial cultures.Further optimization parameters involved suitable carbon sources, suitable nitrogen sources and suitable pH.The best carbon source obtained in Sucrose for S.equisimilis and for N.sicca Lactose was best carbon source. The best nitrogen source obtained inNa2HPO4 for S.equisimilis and for N.sicca. The best growth obtained for S.equisimilis and for N.sicca at pH 7.