Inhibition of green fluorescent protein gene expression in chicken blastoderm cells by siRNA

RNA interference (RNAi) has developed into a powerful tool for studying gene function, embryonic development and virosis therapy which can be capable of silenced the expression of special genes. In order to identify the mechanism of RNAi in the birds, The small interference RNA of GFP (siGFP) and GAPDH (siGAPDH) that synthesized in vitro transcription were cotransfected into the chicken blastoderm cells separately with the green fluorescent protein (GFP) expression vector (pEGFP-C1 Vector) by the transfection reagent (Lipofectamine TM 2000), then the numbers of fluorescent cell were measured after 36 h. The result of variance analysis showed that there were significant differences among the groups(P0.01), and the result of multiple comparison showed that there were significant differences between the groups of GFP and GFP+ siGFP(P0.01), GFP+ siGAPDH and GFP+siGFP(P0.01), but no significant difference between GFP and GFP+ siGAPDH (P0.05). The results indicate that the siGFP can knock-down the expression of GFP but the siGAPDH could not, which means there exists RNAi mechanism in chicken as well as in wireworm, epiphyte, cysticercoid, hydra, trypanosomiasis, turbination, drosophila, zebra, mice, and so on.