Effect of ceramide signaling pathway on anandamide-mediated inhibition of adhesion and invasion of U251 glioma cell line

Objective To observe the effect of ceramide signaling pathway on anandamide (AEA)-mediated inhibition of adhesion and invasion of U251 glioma cell line.Methods Human U251 cells were cultured with DMEM in vitro.Cultured cells were divided into control group,5 μmol/L AEA group,and 5 μmol/L AEA + 10 μmol/L C2-ceramide group.At the same time,the AEA groups were pretreated with 10 μmol/L fumonisin B1 (FB1) for 24 h early.The cell adhesion rate of U251 cells was examined by methyl thiazol tetrazolium (MTT) assay at different time points of AEA treatment.The abilities of invasion were detected by Transwell assay.Results 5 μmol/L AEA,and 5 μmol/L AEA + 10 μmol/L C2-ceramide could inhibit invasive abilities of U251 cells.The capabilities of adhesion and invasion of U251 cells in 5 μmol/L AEA group was reduced by 66.53％ and 83.85％ respectively (P ＜ 0.01),and those in 5 μmol/L AEA + 10 μmol/L C2-ceramide group were decreased by 71.64％ and 86.43％ respectively (P ＜0.01).After pretreatment of U251 cells with 10 μmol/L FB1 for 24 h,The capabilities of adhesion and invasion of U251 cells in 5 μ mol/L AEA group were only decreased by 27.43％ and 32.23％,and those in 5 μmol/L AEA + 10 μmol/L C2-ceramide group decreased by 56.61％ and 68.11％ (P ＜0.01),respectively.Conclusion AEA can inhibit the capabilities of adhesion and invasion of U251 cells via the ceramide signaling de novo synthesis pathway. Key words: Glioma;  Ceramide signaling pathway ;  Invasion