Comparative study on cortical neuron primary culture system and purification in two groups of rats

Objective: To investigate the reliable method of neuron primary culture and purification by comparing the different methods of culture and purification.Methods: Neuron cell of SD rats were separated and cultured in DMEM/F12 serum culture system and Neurobasal/b27 free serum culture system.After culture 3 days,adding arabinosylcytosin and 5fluorodeoxyuridine to purify neuron cell.To observe the morphology of the neuron cells on 1day,3day and 7day by inverted microscope and observe the purity quotient and growth state of neuron cell on 7day by immunofluorescence.Results: morphosis and cell density of neuron cells have no different on 1day and 3day,but cell density of neuron cell by arabinosylcytosin cells is lower than other on 7day,purity quotient of neuron cells in free serum culture system is better than it in serum culture system by immunofluorescence.adding arabinosylcytosin have more influence on growth state of neuron cell than by 5fluorodeoxyuridine.Conclusion: free serum culture system with arabinosylcytosin is a better method of culture and purify neuron cell.