Multisite Recording of Local Field Potentials in Awake, Free-Moving Mice

: Oscillations of local field potentials (LFPs) are crucial in neuroscience studies since they are correlated with many brain activities related to sense, motor, learning, and cognition. Multisite recording of LFPs from different brain areas in awake animals simultaneously is extremely important because they could provide important information on how the brain areas cooperate with each other to perform a specific function. Here, we describe a method that could record LFP signals from six olfactory-related areas (both olfactory bulbs, both piriform cortices, and both hippocampi) in awake free-moving mice. This method could be developed to record up to 16 different brain areas if the shortest distance between any two recording sites is larger than 2 mm.