Quantitative Analysis of Sn-2 Palmitic Acid Content in the Triacylglycerols from Algae Oil Using Enzymatic Hydrolysis

The enzymatic hydrolysis parameters of triacylglycerols (TAGs) in algae oil to get sn-2 glycerel monostearte including substrate amount, lipase amount and reaction time was studied, and the products of fatty acid methyl esters (FAMEs) were detected by gas chromatography (GC). The result indicated that the optimum conditions to hydrolyze TAGs in algae oil were as follows: mass ratio of substrate to lipase of 1:1, 2 mL Tris-HCl buffer, 0.2 mL CaCl2 solution, 0.5 mL sodium cholate hydrate solution were added in each 30mg substrate, and reaction time of 1.5 min with 120 rpm at 40 °C, the highest palmitic acid (PA) content accounted for 9.1% of total oil.