Five New Oxylipins from Chaenomeles sinensis

In the course of our continuing search for bioactive constituents of Korean medicinal plants, we isolated five new oxylipins, chaenomic acid A–E (1–5), and six known ones (6–11) from the twigs of Chaenomeles sinensis. The structures of the new compounds (1–5) were determined by spectroscopic methods, including 1D and 2D NMR (1H and 13C NMR, 1H–1H COSY, HMQC, HMBC, and NOESY), olefin cross-metathesis, and LC/MS analysis. The known compounds (6–11) were identified by comparison of their spectroscopic data and specific optical rotation with the reported data. The isolated compounds (1–11) were tested for their inhibitory effects on nitric oxide production in lipopolysaccharide-activated murine microglial cells and for their cytotoxic activities against four human cancer cell lines (A549, SK-OV-3, A498, and HCT-15).