Transposon Mutagenesis of the Phage φYeO3-12

Transposon insertions were obtained in early and middle regions of the phage genome only, which is consistent with the essential nature of the late genes. Phage genes coding for DNA ligase and lysozyme were found to be needed for efficient propagation of φYeO3-12 in YeO3-c but not in E. coli. Also, in Yersinia but not in other bacteria tested, phage gene 0.45 seemed to be important in the early stages of infection. Still, further studies are needed to understand the biological basis for these phenotypes.