STRmix™ collaborative exercise on DNA mixture interpretation (Accepted Manuscript)
2020
An intra and inter-laboratory study using the probabilistic
genotyping (PG) software STRmix™ is reported.
Two complex mixtures from the PROVEDIt set, analysed on an Applied
Biosystems™ 3500 Series Genetic Analyzer, were selected. 174 participants responded.
For Sample 1 (low template, in the order of 200 rfu for
major contributors) five participants described the comparison as inconclusive
with respect to the POI or excluded him.
Where LRs were assigned, the point estimates ranging from 2 × 104 to 8 ×
106. For Sample 2 (in the order of 2000
rfu for major contributors), LRs ranged from 2 × 1028 to 2 × 1029. Where LRs were calculated, the differences
between participants can be attributed to (from largest to smallest impact):
• varying
number of contributors (NoC),
• the
exclusion of some loci within the interpretation,
• differences in local CE data analysis
methods leading to variation in the peaks present and their heights in the
input files used,
• and
run-to-run variation due to the random sampling inherent to all MCMC-based
methods.
This study demonstrates a high level of repeatability and
reproducibility among the participants.
For those results that differed from the mode, the differences in LR
were almost always minor or conservative.
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