Renal Plasma Membranes: Isolation, General Properties, and Biochemical Components

The sections in this article are: 1 Studies on Isolated Plasma Membranes: Conceptual Framework 2 Isolation of Plasma Membranes of the Proximal Tubule 2.1 Definition of Membranes 2.2 Membrane Marker 2.3 Starting Material 2.4 Isolation of Microvillous Membranes 2.5 Isolation of Basal-lateral Membranes 2.6 Simultaneous Isolation of Microvillous and Basal-lateral Membranes 2.7 Isolation of Endosomes and Reserve Vesicles 3 Purity and General Properties of Plasma Membranes Isolated from the Proximal Tubule 3.1 Microvillous Membranes 3.2 Basal-lateral Membranes 3.3 Endosomes and Reserve Vesicles 4 Isolation of Plasma Membranes of the Thick Ascending limb of Henle's Loop 4.1 Definition of Membranes 4.2 Membrane Marker 4.3 Starting Material 4.4 Methods for Membrane Isolation 5 Purity and General Properties of Plasma Membranes Isolated from the Thick Ascending Limb 5.1 Purity of Membrane Fractions 5.2 General Properties 6 Isolation of Plasma Membranes of the Collecting Duct 6.1 Definition of Membranes 6.2 Membrane Marker 6.3 Starting Material 6.4 Methods for Membrane Isolation 7 Purity and General Properties of Membranes Isolated from the Collecting Duct 8 Biochemical Components of Isolated Renal Plasma Membranes 8.1 Membrane Lipids 8.2 Lipid Composition of Isolated Membranes of the Proximal Tubule 8.3 Lipid Composition of Plasma Membranes Isolated from Kidney Medulla 8.4 Identification of Membrane Proteins 8.5 Enzymes in Renal Membranes 8.6 Antigens in Renal Membranes 8.7 Transport Proteins in Renal Membranes 8.8 Protein Fingerprints of Renal Membranes 9 Concluding Remarks