HPLC Determination of Berberine Chloride in Lianpu Shuangqing Tablet
2006
Objective To determine the berberine chloride content in Lianpu Shuangqing Tablet and to develop a steady and reliable method for the quality control of the tablet.Method A HPLC method was adopted.The column was Dionex C18 (5 μm,4.6 mm ×150 mm)with the column temperature of 28 ℃,the mobile phase was acetonitrile-0.033 mol/L potassium dihydrogen phosphate(25 ∶75) with the flow rate of 1.0 mL/min,and the detection wavelength was at 346 nm.Results Berberine chloride has a linearity in the range of 0.153~1.224 μg,and the average content in the tablet was 1.26 mg/tablet.Conclusion This method is simple,accurate,and can be used for the quality control of Lianpu Shuangqing Tablet.
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