Separation of selected heptacoordinated derivatives of Goshchava-silanates for HPLC.

Optimum conditions of separation and determination was studied of two newly synthesized heptacoordinated benzyl derivatives from the group of Goshchava-silanates: Homo{O,O′,O′′,O′′′′-oxalic acid's Si-(N-benzylaminiomethyl)-Si,Si-dihydroxysilanate} and Homo{O,O′,O′′,O′′′-oxalic acid's Si-(N-benzylaminiomethyl)-Si,Si-diethoxysilanate. In the carried out investigation, we considered three stationary phases (octadecyl, octyl, phenylbutyl) and two mobile phases (acetonitrile and dichloromethane) in various intensities of flow. The best selectivity and the highest separation factor (α = 9.08) was obtained using the mobile phase acetonitrile (100%) and the phenylbutyl column. To reliably optimize the process of separation and determination of the compounds on the phenylbutyl column, the validation of prepared methodology was done.