Original bioactive complexes rich in glycosaminoglycans obtained from small fish

2 S.C. BIOTEHNOS S.A., Otopeni, Romania Abstract An original technology was developed to obtain a bioactive complex from small fish species Engraulis encrassicolus ponticus, Sprattus sprattus sprattus, Odontogadus merlangus euxinus) which contains: glycosaminoglycans, essential amino acids, essential fatty acids (linoleic, linolenic and arachidonic acids), eicosanoids, vitamins (myoinositol), glycerolphosphates, microelements: Ca, Na, K, Fe, Mg, Se, Ni, Cu, Si, etc. The bioactive complex was analyzed by gas chromatography coupled with mass­spectrometry, gel electrophoresis in different systems (SDS­poliacrilamide, cellulose acetate), potentiometric titration, and high performance liquid chromatography. The content of glycosaminoglycans (from which 44% represent sulfated glycosaminoglycans) make the bioactive complex useful to prevent the un unsettlement of the macromolecular structure of background substance and keep the functionality of the extracellular matrix from conjunctive, cartilaginous and bone tissue. Antioxidant activity and regenerative of collagen fibrils was investigated in vitro. The chemical composition provides multiple therapeutically properties: anticoagulant, antithrombotic, antilipidemic, tissue restoration, a strong antioxidant and anti­inflammatory activities. The complex obtained is rich in biological active compounds and could be used to make pharmaceutical preparations with high efficiency and minimal side effects.