Production of Recombinant Proteins from Pichia pastoris: Interfacing Fermentation and Immobilized Metal Ion Affinity Chromatography

The methods describe a Pichia pastoris fermentation system for ­generation and purification of recombinant proteins. The proteins are secreted with hexahistidine tags and purified from feedstock by immobilized metal ion affinity chromatography (IMAC) using either radial flow or expanded bed adsorption. IMAC allows for an initial fast capture and isolation step that omits the need for filtration or centrifugation as primary procedures. The methods are applicable to production of recombinant protein in the laboratory and can be adapted to good manufacturing practice (GMP) compliant processes.