Expression of prostate specific membrane antigen in the neo-vasculature of non-prostate cancers

3110 Background: Prostate specific membrane antigen (PSMA), a transmembrane folate hydrolase consisting of 750 amino acids, has been consistently detected in normal and hyperplastic prostate tissues, in pre-cancerous lesions and prostate cancer (PCa) using immunohistochemistry and other techniques. The expression of PSMA in Non-PCa is currently being pursued as a target for diagnostic imaging and anti-cancer antibody therapeutics. Methods: PSMA expression was measured on a series of fresh frozen and formalin-fixed paraffin embedded non-PCA malignancies by transcriptional profiling (TP) using cDNA microarrays on nylon membranes, RT-PCR (Taqman), in situ hybridization (ISH), western blotting, dual co-localization immunofluorescence (IF) and immunohistochemistry (IHC) both before and after laser capture microdissection (LCM) using both internal domain (7E11) and external domain (MLN591) antibodies. Results: PSMA mRNA expression was localized to the neo-vasculature in 55% of a series of breast, colon, lung an...