Chemical–enzymatic synthesis and bioactivity of mono-6-[Gal-β-1,4-GlcNAc-β-(1,6′)-hexyl]amido-6-deoxy-cycloheptaamylose

Transfer of a β-1,4-galactosyl moiety by galactosyltransferase to chemically synthetised mono-6-(2-acetamido-2-deoxy-β-D-glucopyranosylhexyl)amido-6-deoxy-cycloneptaamylose yields mono-6-[Gal-β-1,4-GlcNAc-β-(1,6′)-hexyl]amido-6-deoxycycloheptaamyiose which shows recognition by the galactose specific Kluveromyces bulgaricus cell wall lectin (Kb CWL)