Low oxygen tension promotes invasive ability and embryo implantation rate

Abstract Low oxygen concentrations during in vitro embryo development not only improving the embryo quality but also can lead to successful implantation. Yet, there is no investigation at the molecular level to indicate the association between increased implantation rate and invasive ability of blastocyst and its inner cell mass quality with in vitro culture under a hypoxic condition. Therefore, the present study was designed to investigate blastocyst formation, total cell number, hatching and implantation rates. In addition we assessed the transcription levels of invasion-( Mmp-9 and uPA ) and pluripotency-related genes ( Pou5f1 , Nanog ) in mouse blastocyst under hypoxic condition. In vivo two-cell embryos were randomly divided into two groups; 5% O 2 and 20% O 2 . Embryos were then cultured to the blastocyst stage and evaluated in terms of cellular parameters. The expression levels of selected genes were also analyzed both in experimental group and in vivo blastocysts recovered from uteri as control group. Results indicated the blastocyst formation, hatching and implantation rates were improved when the embryos were cultured in hypoxic condition. Furthermore, the expression levels of Mmp-9 , Nanog and Pou5f1 showed an increase in 5% O 2 in comparison with 20% O2 group. In conclusion, it seems that hypoxic condition by increasing the quality and invasion ability of the blastocyst can improve implantation rate.