HPLC Determination of Geniposide,Andrographolide and Dehydroandrographolide in Lianzhi Qinghuo Capsules

Objective: To establish an HPLC method for the simultaneous determination of geniposide,andrographolide and dehydroandrographolide in Lianzhi Qinghuo capsules.Method: Geniposide,andrographolide and dehydroandrographolide were separated on ZORBAX SB-C18(4.6 mm ×250 mm,5 μm) column and detected at 254 nm.The mobile phase was methanol-water with gradient elution.The flow rate was 1.0 mL.min-1.Result: Geniposide,andrographolide and dehydroandrographolide were linear within the range of 0.075 8-2.274 0 μg(r = 1),0.046 1-1.383 0 μg(r = 1) and 0.040 9-1.227 0 μg(r = 1) respectively.The average recovery was 99.73%(RSD 1.4%),100.51%(RSD 1.2%) and 101.19%(RSD 1.2%).Conclusion: The method is simple,reproducible,and it can be used for the quality control of Lianzhi Qinghuo capsules.