Table Sugar as Low Cost Option and its Concentration Effect on ShootMultiplication of Sugarcane (Saccharum officinarum L.) Genotypes
2016
Most developing countries are hesitated in maximizing tissue culture technology due to the overhead costs involved. Therefore,
this research was initiated to evaluate alternative cheap carbon sources and energy in culture media in order to reduce production
input costs of sugarcane in vitro propagation. The experiment was carried out in completely randomized design (CRD) with 2 × 6
factorial treatment arrangements of genotypes and carbon source in combination. The interaction analysis of genotypes and table
sugar concentration significantly influenced in vitro sugarcane multiplication. On MS medium with 50 gl-1 table sugar, B4906 gave the
highest (13.42 ± 0.29) shoots, whereas Pr1013 produced a maximum of 7.78 ± 0.19 shoots at 60 gl-1 table sugar. However, 40 gl-1 table sugar was optimum to produce usable and separable shoots for further subculture of multiplication. Accordingly results showed
that table sugar not only enhanced multiplication but also significantly reduced the production input costs by 94.89% when compared
with the analytical grade sucrose.
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