Immunofluorescence Staining of Paraffin Sections Step by Step

Immunofluorescence staining is the most frequently applied technique to detect and visualize various molecules in biological samples. Many protocols can be found in the literature and websites of commercial antibody producers. This can result in time-consuming and costly methodical work to establish a ‘simple’ antibody staining. We here summarize in a stepwise manner an easy-to-follow immunofluorescence staining protocol with improved specific fluorescent signal and reduced background and nonspecific binding signal. This will help scientists to save time, effort and antibody costs during the application of such a valuable technique.