ERG11 polymorphism in voriconazole-resistant Candida tropicalis. Weak role of ERG11 expression, ergosterol content and membrane permeability.

Mutations on ERG11 were detected by gene sequencing and amino acid alignment in 18 Candida tropicalis strains with different degrees of sensibility to VRC. ERG11 expression, sterols content and membrane permeability were also evaluated. We report three missense mutations in ERG11 that resulted in resistance to VRC. The transcriptional levels of ERG11 as well as the ergosterol content and membrane permeability do no demonstrated plenty correlation with the obtained MIC values but a tendency.