Study on the Changes of Bone Marrow CD235a+ Nucleated Red Blood Cell Apoptosis and Signal Pathways in Patients with Chronic Altitude Sickness

This study explored the changes in bone marrow CD235a+ nucleated red blood cell apoptosis and signal pathways in patients with chronic mountain sickness. We collected data from 21 chronic mountain sickness patients and 20 non-chronic mountain sickness patients. Bone marrow mononuclear cells, flow cytometry is used to detect the apoptotic index of CD235a+ nucleated red blood cells and to determine the changes in bone marrow CD235a+ nucleated red blood cell mitochondrial membrane potential; reverse transcription quantitative polymerase chain reaction was used to detect CD235a+ nucleated red blood cells, apoptosis related gene messenger RNA expression level, western blot was used to detect CD235a+ nucleated erythrocyte apoptosis-related protein expression level and compared the indicators between the two groups. Here, we found that the apoptosis of CD235a+ nucleated red blood cells in the bone marrow of chronic mountain sickness patients is negatively correlated with the accumulation of red blood cells. At the same time, the accumulation of red blood cells is positively correlated with the level of hemoglobin. The decrease of CD235a+ nucleated red blood cells in messenger RNA expression levels of apoptosis pathway indicates that the red blood cell apoptosis of chronic mountain sickness patients is related to the change of mitochondrial membrane potential and the reduction of the release of nuclear red blood cell apoptotic protein will also cause the decrease of CD235a+ nucleated red blood cell apoptosis.