Antibacterial and Antioxidant Activity of Coffea benghalensis Roxb.Ex Schult. Fruit against Human Bacteria

Aqueous and solvent extract of fruit of Coffea benghalensis were tested for antibacterial activity against six human bacterial species and antioxidant activity in vitro condition. Among the six bacterial species tested, in aqueous extract Proteus vulgaris recorded a maximum inhibition of 28.0mm at 50µl concentration and least activity was observed in Escherichia coli and recorded 15.0mm inhibition at 50µl concentration. In solvent extract, petroleum ether showed a significant activity against all the test bacterial species and Salmonella typhimurium recorded a maximum antibacterial activity of 31.0mm at 50µl concentration. Petroleum ether extract was followed by chloroform and methanol extract. No activity was observed in Benzene and Ethanol extract. All the results obtained were compared to standard antibiotic Ampicillin and Methicillin. In antioxidant activity, Alcohol water extract recorded a maximum radical scavenging activity of 68.3% at 25µg concentration followed by alcohol extract, Hexane extract, water extract and chloroform extract. Compared to standard antioxidants, ascorbic acid, BHA, and alfa-Tocopherol, fruit extract of C. benghalensis also showed better antioxidant activity.