Non‐neuronal release of ACh plays a key role in secretory response to luminal propionate in rat colon

Colonic chloride secretion is induced by chemical stimuli via the enteric nervous reflex. We have previously demonstrated that propionate stimulates chloride secretion via sensory and cholinergic systems of the mucosa in rat distal colon. In this study, we demonstrate non-neuronal release of ACh in the secretory response to propionate using an Ussing chamber. Mucosa preparations from the colon, not including the myenteric and submucosal plexuses, were used. Luminal addition of propionate and serosal addition of ACh caused biphasic changes in short-circuit current (Isc). TTX (1 μM) had no effects, while atropine (10 μM) significantly inhibited the Isc response to propionate and abolished that to ACh. In response to luminal propionatestimulation,AChwasreleasedintotheserosalfluid.Alinearrelationshipwasobserved between the maximal increase in Isc and the amounts of ACh released 5min after propionate stimulation.ThisAChreleaseinducedbypropionatewasnotaffectedbyatropineandbumetanide, although both drugs significantly reduced the Isc responses to propionate. Luminal addition of 3-chloropropionate, an inactive analogue of propionate, abolished both ACh release and Isc response produced by propionate. RT-PCR analysis indicated that isolated crypt cells from the distal colon expressed an enzyme of ACh synthesis (ChAT) and transporters of organic cation (OCTs), but not neuronal CHT1 and VAChT. The isolated crypt cells contained comparable amounts of ACh to the residual muscle tissues including nerve plexuses. In conclusion, the non-neuronal release of ACh from colonocytes coupled with propionate stimulation plays a key roleinchloridesecretion,viatheparacrineactionofAChonmuscarinicreceptorsofcolonocytes.