Two Novel HPLC Methods which Rapidly Detect the Substrates and Cleavage Products of γ-Glutamyl Hydrolase

γ-Glutamyl hydrolase (EC 3.4.22.12) is widely distributed throughout the phylogenetic spectrum and is thought to play an important role in the cellular homeostasis and nutritional absorption of folates and antifolates1,2. The enzyme catalyzes the endo-or exopeptidyl hydrolysis of the γ-glutamyl side chains of naturally occurring polyglutamylated folates and synthetic antifolates2.