The toolbox of fluorescence standards: flexible calibration tools for the standardization of fluorescence-based measurements
2010
To improve the reliability of fluorescence data in the life and material sciences and to enable accreditation of
fluorescence techniques, standardization concepts are required that guarantee and improve the comparability of
fluorescence measurements. At the core of such concepts are simple and evaluated fluorescence standards for the
consideration of instrument-specific spectral and intensity distortions of measured signals and for instrument
performance validation (IPV). Similarly in need are fluorescence intensity standards for the quantification from
measured intensities and for signal referencing, thereby accounting for excitation light-induced intensity fluctuations.
These standards should be preferably certified, especially for use in regulated areas like medical diagnostics.
This encouraged us to develop liquid and solid standards for different fluorescence parameters and techniques for use
under routine measurement conditions in different formates. Special emphasis was dedicated to the determination and
control of the spectral responsivity of detection systems, wavelength accuracy, homogeneity of illumination, and
intensity referencing for e.g. spectrofluorometers, fluorescence sensors and confocal laser scanning fluorescence
microscopes. Here, we will present design concepts and examples for mono- and multifunctional fluorescence standards
that provide traceability to radiometric units and present a first step towards a toolbox of standards.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
2
Citations
NaN
KQI