Identification and pathogen characteristics analysis of a co-infection with CSFV, PRRSV and PCV2.

[Objective] The aim of this study was to identify swine diseases caused by CSFV,PRRSV and PCV2 and thus to analyze its pathogeny chracteristics.[Method] The tissues and viscera of the diseased swine were collected from Xiangtan of Hunan(Code of HN/XT)to extract DNA and RNA for PCR amplification and sequencing.Meanwhile,the virulent strains were isolated and identified by cell separation technology.[Result] The sequencing analysis results showed that the amino acid homology between CSFV,PRRSV,PCV2 and sequences from current prevalent virulent strains or GenBank was about 90%,94% and 96% respectively.Accordingly,the swine epidemic situation was caused by the mixed infection of three kinds of viruses(CSFV,PRRSV and PCV2)at least.[Conclusion] The research can provide data for the epidemiologic studies and purification control of the mixed infection diseases harmful for the swine breeding industry at present.