EFFECT OF ELECTRON BEAM IRRADIATION ON IMMUNOREACTIVITY AND BIOCHEMICAL PROPERTIES OF PEANUT ALLERGENS

The effects of electron beam(EB) irradiation on allergenicity and biochemical properties of peanut protein were investigated in this study.Peanut protein extracts solution and defatted powder were irradiated at the dose of 2,4,6,8,10,15 and 20kGy.SDS-PAGE electrophoresis was used to observe the change of molecular weight.Changes in allergenicity of irradiated peanut major allergen(Ara h 1,Ara h 2,Ara h 3) were analyzed by immunoblotting and indirect enzyme-linked immunosorbent assay(CI-ELISA).The changes of concentration,turbidity and hydrophobicity of the irradiated protein solution were determined by UV and fluorescence spectrophotometer.Results showed that allergen solution was more sensitive to irradiation than allergen in solid state.When the irradiated dose was lower than 10kGy,the immunoreactivity of allergen solution became little stronger;and when the dose higher than 10kGy,the immunoreactivity was reduced.The IC50 was 11 times than the control,after irradiation at the dose of 20kGy.The concentration and turbidity of the allergen solution increased with the increasing of irradiation dose.Hydrophobicity first went up with increasing irradiation and then decreased when the irradiation dose was above 15kGy.The biochemical properties of peanut allergen were altered and immunoreactivity was reduced by EB irradiation,the effect of electron beam irradiation on peanut allergen in solid state was more significant.