Evaluating the genetic relationship of Lilium species/cultivars based on target region amplification polymorphism (TRAP)

Most of the plant species from the genus Lilium L. have great ornamental and edible value. In this study, target region amplification polymorphism (TRAP) polymorphic marker technique was applied to assess the genetic relationships among 13 Lilium species/cultivars. The technique was also used to evaluate genetic diversity among two population of L. regale E. H. Wilson, cultivar of ‘Regale’ and ‘Regale Album’, and genetic diversity among two population of L. henryi Baker and cultivar of ‘Henryi’. In total, 221 fragments were amplified by 14 pairs of primers for 13 Lilium species/cultivars, of which 59 (26.70%) showed polymorphism. 1160 fragments were amplified for two population of L. regale, cultivar of ‘Regale’ and ‘Regale Album’, of which 943 (81.25%) were polymorphic. 798 fragments were amplified for two population of L. henryi and ‘Henryi’, of which 574 (71.93%) were polymorphic. A cluster analysis was performed based on UPGMA and PCoA by relying on the Nei’s genetic distance. Our results indicated that TRAP marker technique is informative and could be used to evaluate the genetic relationships among Lilium species/cultivars and genetic diversity among populations. The results will also provide more valuable information for Lilium resource protection and current plant breeding programs.