Immobilization of Reducing Oligosaccharides to Matrices by a Glycosylamide Linkage

Abstract A novel method is described for coupling of reducing oligosaccharides to matrices. A one pot, two-step reaction is used, which forms a glycosyl amide bond to the matrix. Unreacted oligosaccharide could easily be recovered. The new matrices were useful in affinity chromatography, e.g., for purification of lectins and antibodies.