RAPID METHOD FOR ISOLATION OF ANDROGRAPHOLIDE FROM AERIAL PARTS OF ANDROGRAPHIS PANICULATA USING MICROWAVE-ASSISTED EXTRACTION AND ITS QUANTIFICATION BY NORMAL PHASE HIGH-PERFORMANCE TLC

Andrographolide (AD) is the most important bioactive phytoconstituent of Andrographis paniculata (Burm.f.) Ness and exhibits antitumor metastasis, anti-HIV, antibacterial, antiinflammatory, antimalarial, antidiabetic activities etc. In this study, microwave-assisted extraction (MAE) technique was employed for rapid isolation of andrographolide from the aerial part of Andrographis paniculata and simultaneously HPTLC was carried out for the quantification of andrographolide in various solvent polarity based extracts. Effects of several experimental parameters on the extraction efficiencies of andrographolide, such as type and volume of extraction solvents, microwave power and extraction times were evaluated. The optimal extraction conditions were found to be 25 mL of a mixture of chloroform/methanol, 50:50; preleaching time, 10 min; microwave power, 450 W; temperature, 50 ο C; and microwave irradiation time, 8 min. Under the optimum conditions, the percentage of andrographolide was quantified to be 0.05 ± 0.021 to 2.24 ± 0.016% in the MAE extracts of aerial parts. Rapidly andrographolide was isolated from the chloroform/methanol MAE extracts with 1.4% yield and 62.5% recovery by precipitation process. The purity of AD was found ≥97.0%. MAE is a faster, convenient, and appropriate