Comparative study of two methods for hepatitis B antigen (HBAG) detection: Double sequential counterimmunoelectrophoresis (DCIEP) and ausria II-125I radioimmunoassay

Abstract Double sequential counterimmunoelectrophoresis (DCIEP) (an improved method that we have developed) and Ausria II- 125 I (an indirect solid-phase radioimmunoassay) were compared for HBAg detection in chronic liver diseases. 26.8% of the patients were positive using DCIEP and 41.8% were positive with Ausria II- 125 I. The conventional counterimmunoelectrophoresis (CIEP) is less sensitive than DCIEP, the level of positive results being 18.8% ( P