Screening and identification of the specific microRNAs involved in the protection of morphine preconditioning against hypoxia/reoxygenation injury in rat failing cardiomyocytes (652.6)

This study was designed to investigate the protective effects of morphine preconditioning (MPC) against hypoxia/reoxygenation (H/R) injury in rat failing cardiomyocytes, and to screen and identify the involved microRNAs (miRNAs). The cardiomyocytes isolated from adriamycin-induced failing rat heart received MPC at different concentrations followed by H/R and then cellular injury was measured. In another experiment, total RNA was extracted from the failing cardiomyocytes after MPC and subjected to miRNA microarray and subsequent bioinfornatics analysis and real-time RT-PCR validation. Typblue exclusion and LDH assay results showed that MPC significantly attenuated cell injury caused by H/R in failing cardiomyocytes. The miRNA microarray data showed that many of miRNAs differentially expressed among groups. By Gene Ontology and KEGG pathways analysis, the predicted target genes were enriched in calcium ion transport, apoptosis and MAPK signal pathway. Notably, miR-133b was obviously downregulated in failing...