The Reference Materials of Preparation about Salmonella Enteritidis

Choose Salmonella enteritidis to the target species, take Chicken mince mixed protectant formula as matrix in Living Bacterium Reference Material, adopt Lyophilization method to develop Living Bacterium Reference Material, for the study of three batches total, each batch prepared the RM containing 100 bottles of Cooked Chicken Matrix and 100 bottles of Raw Chicken Matrix.The results of the uniformity experiment showed that, the repeatability standard deviation of raw chicken matrix and cooked chicken matrix is 2.185 % and 3.596 %,the reproducibility standard deviation is 2.185 % and 3.514 %, the results of the Analysis of Variance are P0.05, it indicated that there was no significant difference between the six batches of RM. Stability experiments showed that the RM stord at-20 ℃, the two kind of RM have good Stability. Conclusion:The Salmonella Enteritidis Living Bacterium Reference Material with corresponding strain properties, good stability, and them have great reference value to study the same type Reference Material, and fill the laboratory testing of quality control contrast and Reference Materials.