RT-PCR based gene expression profiling Clinical quantitation of immune signature in follicular lymphoma by

Abstract Microarray gene expression profiling studies have demonstrated immune response gene signatures which appear predictive of outcome in follicular lymphoma (FL). However, measurement of these marker genes in routine practice remains difficult. We have therefore investigated the immune response in FL using real-time PCR to measure expression levels of 35 candidate Indicator genes, selected from microarray studies, to polyA cDNAs prepared from 60 archived human frozen lymph nodes, in parallel with immunohistochemical analysis for CD3, CD4, CD7, CD8, CD10, CD20, CD21 & CD68. High levels of CCR1, a marker of monocyte activation, were associated with a shorter survival interval, and high levels of CD3 with better survival, whilst immunohistochemistry demonstrated association of high numbers of CD68 positive macrophages with a shorter survival interval and of high numbers of CD7 positive T-cells with a longer survival interval. The results confirm the role of the host immune response in outcome in FL and identify CCR1 as a prognostic indicator and marker of an immune switch between macrophages and a T-cell dominant response. They demonstrate the utility of polyA DNA and real-time PCR for measurement of gene signatures and the applicability of using this type of “molecular block” in clinical practice. From bloodjournal.hematologylibrary.org by guest on June 13, 2013. For personal use only.