Breeding and Characterization of Wheat-Thinopyrum intermedium Alien Substitution Line Shannong 0095

A new wheat germplasm, Shannong0095, was produced from the hybrids of BC2F4 derived from the progeny of Triticum aestivum cultivar, Yannong15, crossing with Thinopyrum intermedium after the hybrid F1 back-crossing with the Yannong 15 two times and three self-crossings. And it was characterized by using morphology, cytology, alcohol soluble protein- poly acrylamide gel electrophoresis (A-PAGE), random amplified polymorphic DNA (RAPD) and genomic in situ hybridization (GISH) analysis. The results were as follows: Shannong 0095 had plant height of 78 cm, ear length of 17.3 cm, number of mean spike of 74, flag leaf length of 36.3 cm, flag leaf width of 3.03 cm, stout haulm, and more flowers and more seeds, which showed that it was more luxuriant than that of Yannong 15. The chromosome number of Shannong 0095 in root tip cell was 2n=42 and its chromosome configuration at PMC MI was 2n=21Ⅱ. Two univalent were observed in most of PMC MI of the hybrids F1 of the crossing between Shannong 0095 and Yannong 15. The mean chromosome configuration of the hybrids F1 was 2n=20.08Ⅱ+1.84Ⅰ. A-PAGE result testified that a specific Thinopyrum intermedium band in ? area was observed in Shannong 0095. Among 180 RAPD primers, the primer S186(5′-GAT ACC TCGG -3′) revealed a specific Thinopyrum intermedium DNA band, about 900bp, that presented in Shannong 0095 and was designed as S186900. The result of GISH with the probe of Thinopyrum intermedium genomic DNA and the blocker of Chinese Spring (C.S) genomic DNA further conformed that Shannong 0095 contains 2 intact chromosomes of Thinopyrum intermedium and is a wheat- Thinopyrum intermedium alien disomic substitution line.