Cloning of Two α-Subunit Genes of ADP-Glucose Pyrophosphorylase from Sweet Potato and Plant Expression Cassettes Construction

ADP-Glucose pyrophosphorylase(AGPase) was the enzyme responsible for the production of adenosine 5'-diphosphase glucose(ADPGlc).The AGPase reaction was the first committed step in the biosynthesis of starch,and AGPase was one of the key enzymes in this pathway.Total RNA was purified from high-starch sweet potato variety Chuanshu 34,and was reverse-transcripted into cDNAs.Two α-subunit genes of AGPase,AGPa1 and AGPa2,were amplified from cDNAs.Afterwards AGPa1 and AGPa2 were sub-cloned into pCambia1301 to construct the recombinant vectors pC-AGPa1 and pC-AGPa2 after bioinformatic analysis.pC-AGPa1 and pC-AGPa2 vectors were introduced into A.tumefaciens strain EHA105,and ready to be used in transformation of sweet potato,potato and cassava.