Forensic application and evaluation of a commercially available pregabalin immunoassay test in serum on an Olympus AU480.

The usability of the commercially available ARK Pregabalin II Assay (ARK Diagnostics) for serum analysis of forensic samples was tested. Overall, 156 samples were tested by both the immunoassay and a validated liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method. Sensitivity was 100%, and specificity was 98.7% (n = 79 positive cases confirmed by LC-MS/MS in a range of 380-37,000 ng/mL).