Determination of monosaccharide composition in polysaccharide of alhagi-honey by pre-column derivatization-high performance capillary electrophoresis

Objective:To determine the monosaccharide composition in polysaccharide from alhagi-honey.Methods:The polysaccharide of alhagi-honey was hydrolyzed with 2 mol·L-1 H2SO4.The monosaccharides reacted with 1-Phenyl-3-methyl-5-pyrazolone(PMP),and the derivatives were separated by HPCE and detected at 245 nm.Results:The molar ratios of arabinose,glucose,rhamnose,galactose,mannose,glucuronic acid,and galacturonic acid in polysaccharide from alhagi-honey were 2.80∶38.61∶5.28∶3.76∶1.91∶3.82∶2.45.The content of glucose in alhagi-honey polysaccharide was 35.65%.Conclusion:The determination method is simple,sensitive,accurate and reliable.The method can be applied in the analysis of monosaccharide composition and quality control of alhagi-honey.