P.1.h.039 Time-course of changes in lipid peroxidation after acute exposure to GBR 12909 in brain areas of mice

behaviour between Nos1-knockout and wild-type animals. In wildtype mice, MS stimulated the locomotor activity in OF (p< 0.05), but increased the anxiety of these animals, as it reduced the time spent exploring the open arm of EPM (p = 0.06). MS had stronger effects on the behaviour of the Nos1-knockout mice. Interestingly, many of the behaviours brought about by the lack of Nos1 − hyperactivity in the Nos1-knockout mice: their behaviour in OF, EPM, and LD was indistinguishable from the wild-type animals. However, MS did not change the impairment of contextual fear learning induced by Nos1-deficiency, and additionally decreased freezing to tone (p< 0.05). Conclusions: These findings reveal an interplay between adverse early life experiences and NO signalling impacting on affective behaviour, suggesting higher stress-sensitivity in animals lacking the neuronal nitric oxide synthase.