Effect of Immobilized Hyaluronidase on Stem and Progenitor Cells in Pulmonary Fibrosis

The effect of immobilized hyaluronidase on stem and progenitor cells of the lungs was studied on the model of partially reversible toxic bleomycin-induced pulmonary fi brosis in C57Bl/6 mice. During the infl ammation phase, immobilized hyaluronidase reduced infi ltration of alveolar interstitium with hemopoietic stem cells Sca-1+, c-Kit+, CD34–, (CD3, CD45R (B220), Ly6C, Ly6G (Gr1), CD11b (Mac1), TER-119)–. Improvement of histological parameters of bleomycin lungs during the phase of collagen fi ber deposition after the treatment was accompanied by accumulation of mesenchymal multipotent stromal cells (CD31–, CD34–, CD45–, CD44+, CD73+, CD90+, CD106+), decrease in the population of pan-hemopoietic cells (CD45+), accelerated restoration of the content of endothelial cells, and inhibition of clonal activity of fi broblast precursors (CD45–).