Microbial community assembly in detergent wastewater treatment bioreactors: Influent rather than inoculum source plays a more important role

Abstract In this study, three sequencing batch reactors Ra, Rb, Rc with different inoculum sources (activated sludge; activated sludge plus detergent degrading consortium; detergent degrading consortium) were used to treat detergent wastewater [consisting of sodium dodecyl sulfate, polyoxyethylene lauryl ether and tetrasodium ethylenediamine tetraacetate (Na 4 EDTA)]. Fast start-up and highest performance in phase I and II (organic loading rate were 0.28, 0.39 kgCOD/kgMLSS/d, respectively) were observed in Rc. In contrast, Rb showed highest impact resistance to the increase of EDTA concentration in phase III. High-throughput sequencing analysis showed that inoculum sources led to significant differences on microbial community in phase I. However, regardless of the influent variation in phases II and III, the differences on microbial community among three SBRs were diminished along long-term operation. Pseudomonas, Sphingopyxis, Luteimonas, Pseudoxanthomonas and SM1A02 were found to be the core taxa, they might contribute to the excellent performance of detergent wastewater treatment.