Hymenobacter jejuensis sp. nov., a UV radiation-tolerant bacterium isolated from Jeju Island

A novel Gram-stain negative, aerobic, rod-shaped, non-motile and pink-coloured bacterium, designated strain 17J68-5T, was isolated from soil in Jeju Island, Korea. The strain was found to grow at 18–37 °C (optimum 25 °C) in R2A medium at pH (6.0 to 7.5; optimum 6.5) in the presence of 0% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 17J68-5T forms a distinct lineage within the family Hymenobacteraceae and is closely related to Hymenobacter daecheongensis DSM 21074T (94.9% 16S rRNA gene sequence similarity), Hymenobacter rutilus K2-33028T (94.6%) and Hymenobacter tibetensis XTM003T (94.3%). The draft genome sequence of strain 17J68-5Tis 5.1 Mb size. The calculated average nucleotide identity and the digital DNA–DNA hybridization between strain 17J68-5T and closely related type strains were 81.3 to 84.1 % and 25.5 to 28.1%. The major cellular fatty acids (≥ 10%) of the strain 17J68-5T were identified as summed feature 3 (C16:1ω6c/C16:1ω7c; 21.2%), iso-C15:0 (19.1%), summed feature 4 (C17:1 iso I/C17:1 anteiso B; 17.9%) and C16:1ω5c (13.1%). The predominant respiratory quinones were found to be menaquinone 7 and 6 (MK-7 and MK-6). The major polar lipid was found to be phosphatidylethanolamine. The genomic DNA G + C content based on the whole genome sequence is 59.6 mol %. The phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate 17J68-5T represents a novel species within the genus Hymenobacter, for which the name Hymenobacter jejuensis sp. nov. is proposed. The type strain of Hymenobacter jejuensis is 17J68-5T (= KCTC 62224T = JCM 33182T).