N,N'-1,10-bis (naringin) triethylenetetraamine, synthesis and as a Cu(II) chelator for Alzheimer's disease therapy.

The bis-Schiff base of N,N-1,10-bis (naringin) triethylenetetraamine (1) was prepared, as a copper (II) ion chelator, compound 1 was used for Alzheimer's disease therapy in vitro. The MTT assay of compound 1 showed that this Schiff base could promote PC12 cells proliferation, and also, compound 1 could inhibit Cu2+-Aβ1-42 mediated cytotoxicity on PC12 cells. The ThT assay showed that 1 can effectively attenuate Cu2+-induced Aβ1-42 aggregation. In addition, compound 1 is determined to be potent antioxidants on the basis of in vitro antioxidant assay, it can effectively decease the level of ROS in Cu2+-Aβ1-42-treated PC12 cells and elevate the SOD activity in Cu2+-Aβ1-42-treated PC12 cells. The results show that N,N-1,10-bis (naringin) triethylenetetraamine is a potential agent for therapy of Alzheimer's disease.