RP-HPLC Method Development and Validation for the simultaneous estimation of Atazanavir sulphate and Ritonavir in bulk and tablet dosage form

A simple, robust, precise and accurate reverse phase liquid chromatographic method was developed for simultaneous estimation of Atazanavir sulphate and Ritonavir in combined tablet dosage form by RP-HPLC method. Chromatography was carried out on a Nucleodur C 18 column (150 mm x 4.6 mm x 5 µm) using Acetonitrile: Methanol: Phosphate buffer, pH was adjusted       to 3.0 with orthophosphoric acid in the ratio of 44:11:45 (V/V) as a mobile phase at a flow rate of 1.5 mL min -1 and eluents were monitored at 210 nm. The calibration curves were linear over the range of 34 – 102 μg mL -1 for Atazanavir sulphate and 10 – 30 μg mL -1 for Ritonavir. The average retention time of Atazanavir sulphate and Ritonavir was found to be 3.133 min and 6.133 min respectively. The method was reproducible, with good resolution of Atazanavir sulphate and Ritonavir. The results of the analysis have been validated statistically.