High Concentration Short Duration Treatment of Benzyladenine Stimulates Adventitious Bud Regeneration from Hypocotyl Explants in Soybean

A new strategy of high concentration short duration treatment of benzyladenine (BA) for enhancing adventitious bud formation from hypocotyl explants were attempted in soybean. It was found that although a regeneration frequency higher than 50% could be achieved by using higher BA concentration, for the induction of possibly more buds while maintaining quality of the buds acceptable, the concentration of BA of 30 mg/l and the treatment duration of 20 min were adopted. Hypocotyls treated only the upper part with BA solution had higher regeneration ability than those treated completely, and hypocotyls geminated on BA free medium had higher regeneration frequency than those geminated on medium containing BA. With the optimized parameters, however, the isolating position on the seedlings for obtaining hypocotyl explants still highly influenced the regeneration efficiency. Three local popular cultivars were tested with little difference in the treating effects, suggesting the validity of this strategy for wider application.