“Characterization of monoclonal antibodies generated to the 287-302 amino acid loop of the human epidermal growth factor receptor”

Background: Dysregulation of EGFR has been implicated in the oncogenesis of various malignancies including glioblastoma and some epithelial cancers. Oncogenesis occurs from overexpression of EGFR, often linked to gene amplification or receptor mutagenesis. The 287-302 loop in the extracellular domain is exposed completely on EGFRvIII, partially exposed on some cancers but cryptic on normal cells. We report on the generation of antibodies to this loop.